Comparative study of low density lipoprotein by estimation and calculation from lipid profile in a tertiary hospital

Lavanya Lagisetty, Radhika Chowdary Darapuneni

Pdf Page Numbers :- 12-16

Lavanya Lagisetty^{1, *} and Radhika Chowdary Darapuneni¹

¹Department Laboratory Medicine, Krishna Institute of Medical Sciences, Minister Road, Secunderabad-500003, Telangana, India

*Corresponding author: Dr. Lavanya Lagisetty, MD (Biochemistry), Department Laboratory Medicine, Krishna Institute of Medical Sciences, Minister Road, Secunderabad-500003, Telangana, India. Email: lavanyamacha99@gmail.com

Received 11 November 2019; Revised 12 December 2019; Accepted 20 December 2019; Published 28 December 2019

Citation: Lagisetty L, Darapuneni RC. Comparative study of low density lipoprotein by estimation and calculation from lipid profile in a tertiary hospital. J Med Sci Res. 2020; 8(1):12-16. DOI: http://dx.doi.org/10.17727/JMSR.2020/8-2

Copyright: © 2020 Lagisetty L et al. Published by KIMS Foundation and Research Center. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Abstract

Introduction: Low-density lipoprotein cholesterol (LDL-C) is widely recognized as an established cardiovascular risk marker. Recent studies have shown that Friedewald underestimates LDL-C at lower levels, which could result in under treatment of high-risk patients of Atherosclerotic cardiovascular disease (ASCVD). A novel method Martin/Hopkins (LDL-CN) using a patient-specific conversion factor provides more accurate calculated LDL-C levels, LDL-C estimation is very important in high-risk patients to consider therapy.

Aims: To compare LDL-C values by using two formulae and direct estimation.

Materials and methods: A prospective study was undertaken in Department of Biochemistry in 1393 subjects from Dec 2018 to March 2019 with mean age of (53.23±13.36). LDL-C was calculated by using Friedewald formula LDL-C (LDL-CF) and Martin/Hopkins equation (LDL-CN) in 1000 subjects with Triglycerides (TG) <400mg/dl and for 393 patients with TG>400 ldl was directly estimated (LDL-CD) with kit in Beckman coulter Dxc860i.

Results: For this analysis, the mean age was (53.23±13.36). In 1000 patients, with TG<400mg/dl the mean LDL-CF (95.13±36.16mg/dl) was significantly lower compared to LDL-CN (98.90±35.88mg/dl) with p value (0.01). Thus, LDL-CN yielded a better diagnostic value than LDL-CF with regression of r2- 0.94. In 393 cases with Tg >400 mg/dl, the mean of LDL-CF (71.83±55.93mg/dl), was significantly lower compared to LDL-CD (104.17±41.6mg/dl) and LDL-CN (107.37±42.73mg/dl). p value was significant between Martin/Hopkins and Friedewald (<0.0001) and it is significant between LDL-CD and LDL-CF (<0.0001) but not between LDL-CD and LDL-CN (0.28).

Conclusion: This data suggest that Martin/Hopkins equation should be the preferred method to calculate LDL-C levels in with TG<400mg/dl and either direct measurement or Martin/Hopkins are the preferred methods in patients with TG>400mg/dl to treat high risk cases of ASCVD.

Keywords: Atherosclerotic cardiovascular disease; ASCVD; triglycerides; low density lipoprotein cholesterol