Analysis of HLA-A, HLA-B and HLA-DRB1 allelic frequencies in tertiary care from Telangana and Andhra Pradesh

Radhika Chowdary D, Sukrutha Gopal Reddy, Reddy VS, Pavani, Prasad Reddy k

Pdf Page Numbers :- 140-144

Radhika Chowdary D^1,*, Sukrutha Gopal Reddy¹, Reddy VS², Pavani¹ and Prasad Reddy K¹

¹Department Laboratory Medicine, Krishna Institute of Medical Sciences, Minister Road, Secunderabad - 500003, Telangana, India

²Department of Nephrology, Krishna Institute of Medical Sciences, Minister Road, Secunderabad - 500003, Telangana, India

*Corresponding author: Dr. D. Radhika chowdary, MD (Biochemistry), Consultant Biochemist & Chief of Labs, Department Laboratory Medicine, Krishna Institute of Medical Sciences, Minister Road, Secunderabad - 500003, Telangana, India. Email: rchowdary13@gmail.com

Received 17 April 2014; Revised 22 May 2014; Accepted 3 June 2014  

Citation: Radhika Chowdary D, Sukrutha Gopal R, Reddy VS, Pavani, Prasad Reddy K. Analysis of HLA-A, HLA-B and HLA-DRB1 allelic frequencies in tertiary care from Telangana and Andhra Pradesh. J Med Sci Res 2014; 2(3):140-144. DOI: http://dx.doi.org/10.17727/JMSR.2014/2-025  

Copyright: © 2014 Radhika Chowdary D et al. Published by KIMS Foundation and Research Center. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Abstract

Introduction: The high polymorphism of the HLA system and its typification is used as valuable tool in establishing association to various illnesses, immune and genetic profiles. It also provides a guide to identify compatibility among donor and recipient of organ transplants.

Objective: To analyse HLA-A, HLA-B and HLA-DRB1 allelic frequencies among renal transplant recipients and their prospective related and unrelated donors attending Nephrology department at KIMS Hospital, Secunderabad.

Methods: 146 kidney recipients and 154 related or unrelated donors coming from different regions of Telangana and Andhra Pradesh for kidney transplantation from September 2007 to December 2013 were included in this study. All were screened for HLA-A, B and DRB, via SSP PCR based methodology and allelic frequency was established.

Result: 1738 and 17 different alleles were identified for HLA-A, B and DRB1 respectively. Alleles found more frequently are A*24, A*02, A*01, B*35, B*61, B*44 and DRB1*15, DRB1*04, DRB1*07.

Conclusion: The results obtained are useful as a reference for organ transplantations and also for allowing compatible donors within specific area for patients requiring renal transplant.   

Keywords: HLA-A; HLA-B; HLA-DRB1; allelic frequencies